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Cell: Dr. Xuri Li and Dr. Peter Carmeliet jointly unveil heterogeneity of endothelial cells across tissues

 

On Feb 13th, 2020, an article titled “Single Cell Transcriptome Atlas of Murine Endothelial Cells” was published by Cell jointly by Dr. Xuri Li at the State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center (ZOC), Sun Yat-Sen University (SYSU) and Dr. Peter Carmeliet at VIB-KU Leuven in Belgium. In this study, Dr. Li and Dr. Carmeliet together with other researchers reveal the heterogeneity of vascular endothelial cells (ECs) across tissues and constructed an EC atlas for the first time, which enables the identification of EC subclusters using public scRNA-seq datasets and thereby provides a powerful discovery tool and resource value.





Dr. Peter Carmeliet (front, middle), Dr. Xuri Li (front, 2nd from left) and some team members at Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou


The heterogeneity of vascular endothelial cells (ECs) across tissues remains incompletely inventoried thus far. In this study, the researchers constructed an atlas of >32,000 single-EC transcriptomes from 11 mouse tissues and identified 78 EC subclusters. It is shown that ECs from brain/testis, liver/spleen, small intestine/colon, and skeletal muscle/heart pairwise express partially overlapping marker genes. Arterial, venous, and lymphatic ECs share more markers in more tissues than does heterogeneous capillary ECs. ECs from different vascular beds, such as arteries, capillaries, veins and lymphatics, exhibit transcriptome similarity across tissues, but the tissue type, rather than the vessel type, contributs to the EC heterogeneity. This EC atlas taxonomy thus will enable researchers to analyze EC subclusters using public scRNA-seq databases and thus will be of significant value to the vascular biology and related fields.




Original article: https://doi.org/10.1016/j.cell.2020.01.015